Application

Anti-SMARCB1 Antibody, clone 2C2 is an antibody against SMARCB1 for use in Western Blotting and Immunocytochemistry.

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected SMARCB1 in 10 µg of HEK293 cell lysate.
Immunocytochemistry Analysis: A representative lot detected SMARCB1 in HeLa cells (Harada, A., et al. (2014). Mono. Antibodies in Immuno. and Immunotherapy. 33:49-51).
Western Blotting Analysis: A representative lot detected SMARCB1 in HeLa cells (Harada, A., et al. (2014). Mono. Antibodies in Immuno. and Immunotherapy. 33:49-51).

General description

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 (SMARCB1), also known as BRG1-associated factor 47 (BAF47) or Integrase interactor 1 protein or SNF5 homolog (hSNF5), and encoded by the gene name SMARCB1 or BAF47, is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein has also been shown to bind to and enhance the DNA joining activity of HIV-1 integrase. It has been found to be a tumor suppressor, and the loss of SMARCB1 has been associated with malignant rhabdoid tumors. Tumor cells of synovial sarcoma seem to have a post transcriptional regulatory mechanism for SMARCB1.

Immunogen

GST-tagged recombinant protein corresponding to human SMARCB1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected SMARCB1 in 10 µg of HeLa cell lysate.

Target description

~ 45 kDa observed